The pervasive deployment of large language models (LLMs) in conversational AI systems has revolutionized information access, yet their propensity for generating factually unsupported or hallucinated responses remains a critical impediment to trustworthiness and widespread adoption. This paper introduces Reinforced Unanswerability Learning (RUL), a novel hybrid training paradigm designed to imbue LLMs with the intrinsic capability to accurately detect unanswerable questions and generate reliably appropriate responses. Unlike conventional approaches that rely on external classifiers or simple prompting, RUL integrates a discriminative unanswerability prediction head with the LLM's generative core, guided by a multi-stage learning strategy. This includes supervised fine-tuning on a novel, richly annotated dataset, Enhanced-CAsT-Answerability (ECA), which features hierarchical answerability labels and ground-truth refusal responses. Crucially, RUL incorporates a subsequent reinforcement learning with human feedback (RLHF) phase to refine the nuance, helpfulness, and informativeness of refusal responses. Extensive experiments demonstrate RUL's superior performance, achieving significantly higher accuracy in unanswerability detection across sentence, paragraph, and ranking levels, and substantially increasing the generation of appropriate refusals for unanswerable queries, alongside strong performance on answerable questions. Human evaluations further corroborate RUL's effectiveness, highlighting a marked improvement in perceived helpfulness and trustworthiness, ultimately paving the way for more reliable and user-centric conversational AI.

Large Language Models (LLMs) have demonstrated remarkable success in diverse natural language processing (NLP) tasks in general domains. However, LLMs sometimes generate responses with the hallucination about medical facts due to limited domain knowledge. Such shortcomings pose potential risks in the utilization of LLMs within medical contexts. To address this challenge, we propose knowledge-tuning, which leverages structured medical knowledge bases for the LLMs to grasp domain knowledge efficiently and facilitate reliable response generation. We also release cMedKnowQA, a Chinese medical knowledge question-answering dataset constructed from medical knowledge bases to assess the medical knowledge proficiency of LLMs. Experimental results show that the LLMs which are knowledge-tuned with cMedKnowQA, can exhibit higher levels of accuracy in response generation compared with vanilla instruction-tuning and offer a new reliable way for the domain adaptation of LLMs.